A Potentially Actionable Suspicious Sample (PASS) is a regulated sample taken from the environment or an APHIS-approved exempt facility2 in which preliminary diagnostics indicate the presence of the USDA-regulated pathogen Candidatus Liberibacter asiaticus. indicated and must be confirmed by APHIS Plant Pathogen Confirmation Diagnostic. Research Institute (PPCDL).
Samples taken from unregulated areas
APHIS-approved testing for the presence of Candidatus Liberibacter asiaticus by a National Plant Protection Laboratory Accreditation Program (NPPLAP)-accredited laboratory4 using APHIS-approved testing equipment collected from non-regulated areas3 or APHIS-approved exempt facilities. A regulatory sample that tested positive. The screening test is considered passed and must be forwarded to PPCDL for final determination. Sample 5 must consist of symptomatic plant material.
sample diagnosis
USDA APHIS PPQ Science and Technology
Plant pathogen identification and diagnosis laboratory
9901 Powder Mill Road Building 580
Laurel, MD 20708
Phone: (301) 313-9208 or (301) 313-9271
If you wish to transfer materials, please notify the laboratory via email (APHIS-PPQCPHSTBeltsvilleSampleDiagnostics@usda.gov). This communication will include sample numbers, screening diagnostic results, and follow-up information. Please do not ship samples on Fridays or the day before federal holidays.
See additional information for sample preparation and submission.
Samples taken from regulated areas
Regulatory samples collected in Regulatory Area 6 in which a NPPLAP-accredited laboratory shows a preliminary diagnosis of Candidatus Liberibacter asiaticus using an APHIS-approved screening test will be considered a PASS and will be forwarded to the PPCDL only under the following conditions: Must be.
The sample is an unusual detection (new condition or new host) and/or the sample may cause an expansion of a previously established regulatory area
NPPLAP-certified analysts at NPPLAP-certified laboratories will be able to collect regulated samples from regulated areas (previously confirmed positive by APHIS) if the laboratory notifies the APHIS national or regional program manager of a positive test within 24 days. authorized to make final decisions regarding Diagnosis results take many hours.
definition
Regulatory Sample: This is a sample of regulatory concerns collected by regulatory agencies to APHIS regarding citrus greening/huanglongbin.
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APHIS Approved Exempted Facility: A greenhouse structure designed to exclude quarantine pests that has been approved by APHIS to produce citrus seedlings for interstate transportation.
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Unregulated Areas: Areas in the United States where citrus canker is not known to occur.
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NPPLAP Certified Laboratory: APHIS through the National Plant Protection Laboratory Accreditation Program (NPPLAP) as possessing all equipment and certified personnel necessary to conduct citrus greening screening tests in accordance with current APHIS work instructions. Institute recognized by.
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Sample: A sample is a single bag of leaves or a twig with leaves on it. Each sample must contain at least 12 symptomatic (unhealthy) leaves. Preferably 12 to 16 leaves. It is highly recommended that each sample be from one plant.
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Regulated Areas: APHIS-certified areas where citrus greening/huanglongbing is federally confirmed.
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